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Scientific Imaging with Photoshop: Methods, Measurement, and Output by Jerry Sedgewick

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Chapter 4. Getting the Best Input

Getting the Best Input

Image of stained section (Carolina Biological Supply, Burlington, NC) from an onion using a 63x, 1.4 N.A. objective on a Zeiss Axiovert 2. Image was acquired with a Spot 1 camera using Spot 2.1 software (Diagnostic Instruments, Sterling Heights, MI). Photoshop CS3 Extended, version 10.1 (Adobe Systems Incorporated, San Jose, CA), was used to conform image to publication by sharpening and minimally correcting color; and corrected for chromatic aberration with the Reduce Noise filter. Scale bar = 20 microns.

VISUAL DATA INTENDED for any output contains as much detail as possible within the limits of the acquisition ...

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