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Progenitor and Stem Cell Technologies and Therapies by A Atala

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1

Basic principles for stem cell culture

J. Davis,     University of Hertfordshire, UK

Abstract:

There are six basic principles that guide cell culture practice and help ensure the quality of the work, the communication of its results, and the safety of all concerned. These are detailed, explained and expanded in this chapter, and are accompanied by examples, illustrations and tips.

Key words

cell culture

Good Cell Culture Practice

standardization

quality assurance

safety

1.1 Introduction

Although often perceived as being one of the more difficult and demanding areas of cell culture, the in vitro growth, maintenance, manipulation, differentiation and use of progenitor and stem cells is subject to the same basic principles, constraints ...

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