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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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6.5. Pixel (Photosite) Size and Resolution

When doing research work, the photomicrographer must remember that the camera is a sampling device that can limit the microscope's ability to reveal fine detail. This is evident when work requires recording the finest details at the limit of lens performance. With too little magnification, fine details are obscured because the individual pixels are larger than the details they are trying to capture. Therefore, all of the detail falls on a single photosite.

There are two requirements for distinguishing objects as separate or resolved. To detect two objects as being separate, each one must fall on a different photosite. Additionally, these photosites must be separated by photo-sites where the object is ...

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