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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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7.8. Manual Exposure

Digital SLRs have an automatic exposure control that can vary the f-stop of the lens and the shutter speed of the camera. Either the aperture varies while the shutter speed is held constant (shutter preferred) or the shutter speed varies while the aperture is held constant (aperture preferred). Many cameras have a Program mode in which the camera attempts to adjust both the aperture and shutter speed. This automated setting should be avoided.

Figure 7.9. A useful control for judging correct exposure is the clip detector. When the photosites are saturated with light, the camera indicates that they are overexposed. In this photograph, the red areas, i.e., the regions with no tissue, are overexposed. It is permissible to overexpose ...

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