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Methods in Molecular Biophysics

Book Description

Our knowledge of biological macromolecules and their interactions is based on the application of physical methods, ranging from classical thermodynamics to recently developed techniques for the detection and manipulation of single molecules. These methods, which include mass spectrometry, hydrodynamics, microscopy, diffraction and crystallography, electron microscopy, molecular dynamics simulations, and nuclear magnetic resonance, are complementary; each has its specific advantages and limitations. Organised by method, this textbook provides descriptions and examples of applications for the key physical methods in modern biology. It is an invaluable resource for undergraduate and graduate students of molecular biophysics in science and medical schools, as well as research scientists looking for an introduction to techniques beyond their specialty. As appropriate for this interdisciplinary field, the book includes short asides to explain physics aspects to biologists and biology aspects to physicists.

Table of Contents

  1. Coverpage
  2. Methods in Molecular Biophysics
  3. Dedication
  4. Title page
  5. Copyright page
  6. Dedication
  7. Contents
  8. Foreword by D. M. Engelman
  9. Foreword by Pierre Joliot
  10. Preface
  11. Introduction Molecular biophysics at the beginning of the twenty-first century: from ensemble measurements to single-molecule detection
  12. Part A Biological macromolecules and physical tools
    1. Chapter A1 Macromolecules in their environment
    2. Chapter A2 Macromolecules as physical particles
    3. Chapter A3 Understanding macromolecular structures
  13. Part B Mass spectrometry
    1. Chapter B1 Mass and charge
    2. Chapter B2 Structure function studies
  14. Part C Thermodynamics
    1. Chapter C1 Thermodynamic stability and interactions
    2. Chapter C2 Differential scanning calorimetry
    3. Chapter C3 Isothermal titration calorimetry
    4. Chapter C4 Surface plasmon resonance and interferometry-based biosensors
  15. Part D Hydrodynamics
    1. Chapter D1 Biological macromolecules as hydrodynamic particles
    2. Chapter D2 Fundamental theory
    3. Chapter D3 Macromolecular diffusion
    4. Chapter D4 Analytical ultracentrifugation
    5. Chapter D5 Electrophoresis
    6. Chapter D6 Electric birefringence
    7. Chapter D7 Flow birefringence
    8. Chapter D8 Fluorescence depolarisation
    9. Chapter D9 Viscosity
    10. Chapter D10 Dynamic light scattering
    11. Chapter D11 Fluorescence correlation spectroscopy
  16. Part E Optical spectroscopy
    1. Chapter E1 Visible and IR absorption spectroscopy
    2. Chapter E2 Two-dimensional IR spectroscopy
    3. Chapter E3 Raman scattering spectroscopy
    4. Chapter E4 Optical activity
  17. Part F Optical microscopy
    1. Chapter F1 Light microscopy
    2. Chapter F2 Atomic force microscopy
    3. Chapter F3 Fluorescence microscopy
    4. Chapter F4 Single-molecule detection
    5. Chapter F5 Single-molecule manipulation
  18. Part G X-ray and neutron diffraction
    1. Chapter G1 The macromolecule as a radiation scattering particle
    2. Chapter G2 Small-angle scattering
    3. Chapter G3 X-ray and neutron macromolecular crystallography
  19. Part H Electron diffraction
    1. Chapter H1 Electron microscopy
    2. Chapter H2 Three-dimensional reconstruction from two-dimensional images
  20. Part I1 Molecular dynamics
    1. Chapter I1 Energy and time calculations
    2. Chapter I2 Neutron spectroscopy
  21. Part J Nuclear magnetic resonance
    1. Chapter J1 Frequencies and distances
    2. Chapter J2 Experimental techniques
    3. Chapter J3 Structure and dynamics studies
  22. References
  23. Index of eminent scientists
  24. Subject index