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Essentials of Molecular Biology

Book Description

Molecular Biology provides an introduction to the concepts of molecular biology in strict adherence to the UGC curriculum for undergraduate students of biochemistry, microbiology, biotechnology, bioinformatics, botany and zoology offered by all Indian universities. Replete with vivid illustrations, the book probes the recent developments in epigenetics, drug discovery, genomics proteomics, prions and oncology. Exhaustive coverage of the fundamentals of molecular biology as well as comprehensive review questions and multiple-choice questions make this book a perfect text for classroom.

Table of Contents

  1. Cover
  2. Title Page
  3. Contents
  4. Dedication
  5. List of Illustrations
  6. Preface
  7. 1. Nucleic Acids
    1. Introduction
    2. DNA and RNA as Genetic Materials
    3. Structure of Nucleic Acids
    4. Properties of the DNA
    5. DNA Topology
    6. Types of RNA
    7. Types of DNA
    8. DNA as Vehicle of Inheritance
    9. Summary
    10. Review Questions
    11. References
  8. 2. Gene Organization
    1. Introduction
    2. Summary
    3. Review Questions
    4. References
  9. 3. DNA Replication
    1. Introduction
    2. Chemistry of DNA Synthesis
    3. Modes of DNA Replication
    4. Semi-conservative Mode of Replication/Meselson and Stahl Experiment
    5. Enzymes of Replication
    6. Models of Replication
    7. Prokaryotic Replication
    8. Eukaryotic Replication
    9. Inhibitors of Replication
    10. Summary
    11. Review Questions
    12. References
  10. 4. Transcription
    1. Introduction
    2. Differences Between Replication and Transcription
    3. Chemistry of Transcription
    4. RNA Polymerase of Prokaryotes
    5. The Sigma Subunit
    6. Promoters
    7. Prokaryotic Transcription
    8. Eukaryotic RNA Polymerases
    9. Transcriptional Events of Eukaryotic RNA
    10. Post-transcriptional Modifications
    11. Inhibitors of RNA Metabolism
    12. Reverse Transcription
    13. Summary
    14. Review Questions
    15. References
  11. 5. Translation
    1. Introduction
    2. Genetic Code
    3. Deciphering the Genetic Code
    4. Characteristic Features of the Genetic Code
    5. Wobble Hypothesis
    6. Ribosome Structure
    7. Protein Synthesis in Prokaryotes
    8. Protein Synthesis in Eukaryotes
    9. Post-translational Modifications
    10. Summary
    11. Review Questions
    12. References
  12. 6. Protein Sorting
    1. Introduction
    2. Signal Sequences
    3. Translocation of Secretory Proteins Across the ER
    4. Protein Modifications in the ER
    5. Protein Targeting to Mitochondria and Chloroplast
    6. Protein Targeting to the Nucleus
    7. Receptor-Mediated Endocytosis and Sorting of Internalized Proteins
    8. Inhibitors of Protein Synthesis
    9. Summary
    10. Review Questions
    11. References
  13. 7. Gene Regulation
    1. Introduction
    2. Regulation of Gene Expression in Prokaryotes
    3. Induction and Repression
    4. Types of Operons
    5. Lac Operon
    6. Regulation of Lactose Operon
    7. Catabolite Repression
    8. Positive Regulation of Lac Operon
    9. Tryptophan Operon and Attenuation
    10. Regulation of Eukaryotic Gene Expression
    11. Regulation of the Chromatin Structure
    12. Regulation of Gene Expression at Transcription Level
    13. The DNA-Binding Domains of the Regulatory Proteins Involved in Transcriptional Control
    14. Regulatory Proteins also have Protein–Protein Interaction Domain
    15. Regulation of Gene Expression by Hormone Action
    16. Summary
    17. Review Questions
    18. References
  14. 8. Gene Mutation and DNA Repair
    1. Introduction
    2. Types of DNA Damages
    3. Mutations and Types
    4. Physical and Chemical Mutagens
    5. Chromosomal Aberrations
    6. Repair of DNA
    7. Oncogenes
    8. Summary
    9. Review Questions
    10. References
  15. 9. Recombinant DNA Technology
    1. Introduction
    2. DNA Isolation and Sequencing
    3. Tools of Recombinant DNA Technology
    4. Restriction Endonucleases
    5. Producing rDNA By Sticky-End Ligation
    6. Producing rDNA By Blunt-End Ligation
    7. Other Enzymes Used in rDNA Technology
    8. Cloning Vectors
    9. Nucleic Acid Hybridization and Probes
    10. In Situ Hybridization
    11. Molecular Cloning
    12. Construction of C-DNA and Genomic Libraries
    13. Applications of Recombinant DNA Technology
    14. Summary
    15. Review Questions
    16. References
  16. 10. Epigenetics
    1. Introduction
    2. Heterochromatin and Histone Interactions
    3. Polycomb and Trithorax
    4. CpG Islands
    5. Genomic Imprinting
    6. Epigenetic Effects and Inheritance
    7. Prions
    8. Summary
    9. Review Questions
    10. References
  17. 11. Genomics and Proteomics
    1. Introduction
    2. Genomics
    3. Structural Genomics
    4. Functional Genomics
    5. Microarray or Gene Chip
    6. Proteomics
    7. Classification of Proteomics
    8. Proteomics Tools
    9. Proteomics and Drug Discovery
    10. Summary
    11. Review Questions
    12. References
  18. 12. Techniques in Molecular Biology
    1. Introduction
    2. Gel Electrophoresis
    3. Blotting Techniques
    4. Restriction Digestion and Ligation
    5. Random Amplification of Polymorphic DNA
    6. Polymerase Chain Reaction
    7. Summary
    8. Review Questions
    9. References
  19. Acknowledgements
  20. Copyright