Because the reaction of urea and urease is carried out in aqueous solution, water is, of course, in excess, and the concentration of water is therefore considered constant. Let
Dividing the numerator and denominator of Equation (9-24) by k1, we obtain a form of the Michaelis–Menten equation:
The final form of the rate law
The parameter kcat is also referred to as the turnover number. It is the number of substrate molecules converted to product in a given time on a single-enzyme molecule when the enzyme is ...