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Bioprocess Engineering

Book Description

Biotechnology is an expansive field incorporating expertise in both the life science and engineering disciplines. In biotechnology, the scientist is concerned with developing the most favourable biocatalysts, while the engineer is directed towards process performance, defining conditions and strategies that will maximize the production potential of the biocatalyst. Increasingly, the synergistic effect of the contributions of engineering and life sciences is recognised as key to the translation of new bioproducts from the laboratory bench to commercial bioprocess. Fundamental to the successful realization of the bioprocess is a need for process engineers and life scientists competent in evaluating biological systems from a cross-disciplinary viewpoint. Bioprocess engineering aims to generate core competencies through an understanding of the complementary biotechnology disciplines and their interdependence, and an appreciation of the challenges associated with the application of engineering principles in a life science context. Initial chapters focus on the microbiology, biochemistry and molecular biology that underpin biocatalyst potential for product accumulation. The following chapters develop kinetic and mass transfer principles that quantify optimum process performance and scale up. The text is wide in scope, relating to bioprocesses using bacterial, fungal and enzymic biocatalysts, batch, fed-batch and continuous strategies and free and immobilised configurations.

  • Details the application of chemical engineering principles for the development, design, operation and scale up of bioprocesses
  • Details the knowledge in microbiology, biochemistry and molecular biology relevant to bioprocess design, operation and scale up
  • Discusses the significance of these life sciences in defining optimum bioprocess performance

Table of Contents

  1. Cover image
  2. Title page
  3. Table of Contents
  4. Copyright
  5. Dedication
  6. List of figures
  7. List of plates
  8. Preface
  9. About the author
  10. Chapter 1: Historical development: from ethanol to biopharmeceuticals
    1. Abstract:
  11. Chapter 2: Microbiology
    1. Abstract:
    2. 2.1 Microorganisms: the core of cellular bioprocesses
    3. 2.2 Cellular structure and sites of metabolic reactions
    4. 2.3 Classification according to carbon and energy requirements
    5. 2.4 Nutrient requirements
  12. Chapter 3: Metabolic macromolecules
    1. Abstract:
    2. 3.1 Carbohydrates
    3. 3.2 Lipids
    4. 3.3 Proteins
    5. 3.4 Nucleosides, nucleotides and nucleic acids
  13. Chapter 4: Molecular biology
    1. Abstract:
    2. 4.1 Replication, transcription and translation
    3. 4.2 Genetic regulation
    4. 4.3 Genetic modification
  14. Chapter 5: Carbon metabolism
    1. Abstract:
    2. 5.1 Energy generation, storage and transfer
    3. 5.2 Catabolic pathways: energy generation
    4. 5.3 Anabolic pathways: energy utilisation
  15. Chapter 6: Enzymes as biocatalysts
    1. Abstract:
    2. 6.1 Enzyme kinetics with no inhibition
    3. 6.2 Enzyme kinetics with inhibition
    4. 6.3 Enzyme reactors with soluble enzymes
    5. 6.4 Enzyme reactors with immobilised enzymes
  16. Chapter 7: Microbial kinetics during batch, continuous and fed-batch processes
    1. Abstract:
    2. 7.1 The nutrient medium
    3. 7.2 Batch process design equations
    4. 7.3 Continuous process design equations
    5. 7.4 Fed-batch bioprocess design equations
  17. Chapter 8: The oxygen transfer rate and overall volumetric oxygen transfer coefficient
    1. Abstract:
    2. 8.1 Oxygen transfer design equations
    3. 8.2 Measurement of the oxygen transfer rate
  18. Chapter 9: Bioprocess scale up
    1. Abstract:
    2. 9.1 Scale up with constant oxygen transfer rate
    3. 9.2 Scale up with constant mixing
    4. 9.3 Scale up with constant shear stress
    5. 9.4 Scale up with constant flow regime
  19. Chapter 10: Bioprocess asepsis and sterility
    1. Abstract:
    2. 10.1 Heat sterilisation of media and equipment
    3. 10.2 Filter sterilisation of air
  20. Chapter 11: Downstream processing
    1. Abstract:
    2. 11.1 Overview of potential recovery operations
    3. 11.2 Separation of cells and extracellular fluid
    4. 11.3 Cell rupture and separation of cell extract
    5. 11.4 Concentration and purification of soluble products
  21. Index