Next-generation sequencing (NGS) is one of the fundamental technological developments of the decade in life sciences. Whole genome sequencing (WGS), RAD-Seq, RNA-Seq, Chip-Seq, and several other technologies are routinely used to investigate important biological problems. These are also called high-throughput sequencing technologies, and with good reason: they generate vast amounts of data that needs to be processed. NGS is the main reason that computational biology has become a big-data discipline. More than anything else, this is a field that requires strong bioinformatics techniques.
Here, we will not discuss each individual NGS technique per se (this would require a whole book on its own). We will use an existing WGS dataset ...
