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Bacteriophage Tail Fibers as a Basis for Structured Assemblies by Timothy Harrah Harrah, Paul Hyman

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4. Tail fiber modifications

We and others working in the Goldberg lab have succeeded in identifying or introducing a number of changes into tail fiber proteins. These changes include deletions and insertions, and epitopes or other peptides as attachment sites for other molecules. For the following sections all bacteria and phage strains are listed in Appendix F, growth media and other buffers are described in Appendix G and general stock preparation and mutant generation methods are described in Appendix H.

4.1 Deletions and insertions

In one project, we set out to introduce an engineered deletion into the wild-type gp 37 which makes up the distal part of the long tail fiber (LTF). This was done by cloning a segment of gene 37 into a plasmid ...

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