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Bacteriophage Tail Fibers as a Basis for Structured Assemblies by Timothy Harrah Harrah, Paul Hyman

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3. Tail fiber production and purification

While much of our work was focused on altering tail fiber proteins to enhance existing properties or add novel ones, we also addressed the issue of large scale production of assembled tail fibers. Several methods for the purification of the long tail fiber and its component half fibers in phage infected lysates have been reported previously (Cerritelli et al., 1996; Earnshaw et al., 1979; Imada and Tsugita, 1970; Imada and Tsugita, 1972; Selivanov et al., 1987; Ward and Wood, 1968; Ward et al., 1970). However, each of these methods for LTF purification relied on serial column based chromatographic steps that can be complex, expensive and relatively inefficient. As our area of interest revolved around ...

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