A number of researchers have found that the most tweets are sent on Tuesdays, Wednesdays and Thursdays. While that may sound like a high-traffic time to avoid, it turns out that's when the most retweets are sent, too, suggesting those are the days when people are most likely to pay attention to your messages.
Along the same lines, for maximum exposure in the U.S., send your messages during Eastern Time business hours.
The chart here, from HubSpot's late 2008 "State of the Twittersphere" report, shows the mid-week pattern. 2009 research has confirmed the trend. (For the full report, see http://blog.hubspot.com/blog/tabid/6307/bid/4439/State-of-the-Twittersphere-Q4-2008-Report.aspx.)
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